mdck ii american type culture collection cells Search Results


mdck-ii cells ecacc  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures mdck-ii cells ecacc
Mdck Ii Cells Ecacc, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mdck-ii cells ecacc - by Bioz Stars, 2026-03
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madin-darby canine kidney (mdck) cells  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection madin-darby canine kidney (mdck) cells
HM/06 replicated efficiently in U251 cells and induced apoptosis in U251; ( A ) U251 cells were infected by HM/06 at MOI 1.0, and cells were lysed for NP detecting by western blot at indicated times post-infection; ( B ) HM/06-infected U251 cells were fixed, permeabilized, and stained with corresponding antibodies for immunofluorescence observation with an OLYMPUSIX70microscope. Bar, 50 µm; ( C ) U251 cells were infected by HM/06 at MOI 1.0, and at indicated times post-infection, supernatants were collected and viruses titer were determined by TCID 50 in <t>MDCK</t> cells. Data was showed as means ± SEM from three experiments; ( D ) U251 cells were infected by HM/06, and at 6, 12, and 24hpi, cells were washed with PBS, detached from the culture plates. Apoptosis was detected using FITC-Annexin V Apoptosis Detection Kit. Data were presentedas mean ± SEM from three experiments. Statistical significance was analyzed by student’s t -test. * p < 0.05, *** p < 0.001.
Madin Darby Canine Kidney (Mdck) Cells, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/madin-darby canine kidney (mdck) cells/product/China Center for Type Culture Collection
Average 90 stars, based on 1 article reviews
madin-darby canine kidney (mdck) cells - by Bioz Stars, 2026-03
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mdck cells  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection mdck cells
HM/06 replicated efficiently in U251 cells and induced apoptosis in U251; ( A ) U251 cells were infected by HM/06 at MOI 1.0, and cells were lysed for NP detecting by western blot at indicated times post-infection; ( B ) HM/06-infected U251 cells were fixed, permeabilized, and stained with corresponding antibodies for immunofluorescence observation with an OLYMPUSIX70microscope. Bar, 50 µm; ( C ) U251 cells were infected by HM/06 at MOI 1.0, and at indicated times post-infection, supernatants were collected and viruses titer were determined by TCID 50 in <t>MDCK</t> cells. Data was showed as means ± SEM from three experiments; ( D ) U251 cells were infected by HM/06, and at 6, 12, and 24hpi, cells were washed with PBS, detached from the culture plates. Apoptosis was detected using FITC-Annexin V Apoptosis Detection Kit. Data were presentedas mean ± SEM from three experiments. Statistical significance was analyzed by student’s t -test. * p < 0.05, *** p < 0.001.
Mdck Cells, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mdck cells/product/China Center for Type Culture Collection
Average 90 stars, based on 1 article reviews
mdck cells - by Bioz Stars, 2026-03
90/100 stars
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mdck cells gdc0012  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection mdck cells gdc0012
HM/06 replicated efficiently in U251 cells and induced apoptosis in U251; ( A ) U251 cells were infected by HM/06 at MOI 1.0, and cells were lysed for NP detecting by western blot at indicated times post-infection; ( B ) HM/06-infected U251 cells were fixed, permeabilized, and stained with corresponding antibodies for immunofluorescence observation with an OLYMPUSIX70microscope. Bar, 50 µm; ( C ) U251 cells were infected by HM/06 at MOI 1.0, and at indicated times post-infection, supernatants were collected and viruses titer were determined by TCID 50 in <t>MDCK</t> cells. Data was showed as means ± SEM from three experiments; ( D ) U251 cells were infected by HM/06, and at 6, 12, and 24hpi, cells were washed with PBS, detached from the culture plates. Apoptosis was detected using FITC-Annexin V Apoptosis Detection Kit. Data were presentedas mean ± SEM from three experiments. Statistical significance was analyzed by student’s t -test. * p < 0.05, *** p < 0.001.
Mdck Cells Gdc0012, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mdck cells gdc0012/product/China Center for Type Culture Collection
Average 90 stars, based on 1 article reviews
mdck cells gdc0012 - by Bioz Stars, 2026-03
90/100 stars
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continuous mdck cells  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection continuous mdck cells
HM/06 replicated efficiently in U251 cells and induced apoptosis in U251; ( A ) U251 cells were infected by HM/06 at MOI 1.0, and cells were lysed for NP detecting by western blot at indicated times post-infection; ( B ) HM/06-infected U251 cells were fixed, permeabilized, and stained with corresponding antibodies for immunofluorescence observation with an OLYMPUSIX70microscope. Bar, 50 µm; ( C ) U251 cells were infected by HM/06 at MOI 1.0, and at indicated times post-infection, supernatants were collected and viruses titer were determined by TCID 50 in <t>MDCK</t> cells. Data was showed as means ± SEM from three experiments; ( D ) U251 cells were infected by HM/06, and at 6, 12, and 24hpi, cells were washed with PBS, detached from the culture plates. Apoptosis was detected using FITC-Annexin V Apoptosis Detection Kit. Data were presentedas mean ± SEM from three experiments. Statistical significance was analyzed by student’s t -test. * p < 0.05, *** p < 0.001.
Continuous Mdck Cells, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/continuous mdck cells/product/China Center for Type Culture Collection
Average 90 stars, based on 1 article reviews
continuous mdck cells - by Bioz Stars, 2026-03
90/100 stars
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HM/06 replicated efficiently in U251 cells and induced apoptosis in U251; ( A ) U251 cells were infected by HM/06 at MOI 1.0, and cells were lysed for NP detecting by western blot at indicated times post-infection; ( B ) HM/06-infected U251 cells were fixed, permeabilized, and stained with corresponding antibodies for immunofluorescence observation with an OLYMPUSIX70microscope. Bar, 50 µm; ( C ) U251 cells were infected by HM/06 at MOI 1.0, and at indicated times post-infection, supernatants were collected and viruses titer were determined by TCID 50 in MDCK cells. Data was showed as means ± SEM from three experiments; ( D ) U251 cells were infected by HM/06, and at 6, 12, and 24hpi, cells were washed with PBS, detached from the culture plates. Apoptosis was detected using FITC-Annexin V Apoptosis Detection Kit. Data were presentedas mean ± SEM from three experiments. Statistical significance was analyzed by student’s t -test. * p < 0.05, *** p < 0.001.

Journal: Viruses

Article Title: Insights into Human Astrocyte Response to H5N1 Infection by Microarray Analysis

doi: 10.3390/v7052618

Figure Lengend Snippet: HM/06 replicated efficiently in U251 cells and induced apoptosis in U251; ( A ) U251 cells were infected by HM/06 at MOI 1.0, and cells were lysed for NP detecting by western blot at indicated times post-infection; ( B ) HM/06-infected U251 cells were fixed, permeabilized, and stained with corresponding antibodies for immunofluorescence observation with an OLYMPUSIX70microscope. Bar, 50 µm; ( C ) U251 cells were infected by HM/06 at MOI 1.0, and at indicated times post-infection, supernatants were collected and viruses titer were determined by TCID 50 in MDCK cells. Data was showed as means ± SEM from three experiments; ( D ) U251 cells were infected by HM/06, and at 6, 12, and 24hpi, cells were washed with PBS, detached from the culture plates. Apoptosis was detected using FITC-Annexin V Apoptosis Detection Kit. Data were presentedas mean ± SEM from three experiments. Statistical significance was analyzed by student’s t -test. * p < 0.05, *** p < 0.001.

Article Snippet: Human astrocyte cells U251 and Madin-Darby canine kidney (MDCK) cells were obtained from China Center for Type Culture Collection (CTCC, Wuhan, China),maintained in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10% heat-inactivated fetal bovine serum (FBS) (HyClone, Logan, UT, USA), and incubated in 37 °C humidified incubator with 5% CO 2 .A/duck/Hubei/hangmei01/2006(H5N1) (HM/06) isolated from duck brain tissues[ ], which can cause neurovirulence and mortality in ducks, was propagated in nine-day-old specific pathogen-free embryonated eggs and titrated in MDCK cells by common plaque assays.

Techniques: Infection, Western Blot, Staining, Immunofluorescence